Regulatory
Pcpc560

Part:BBa_K2924000:Design

Designed by: Andreas Nakielski   Group: iGEM19_Duesseldorf   (2019-10-11)


Pcpc560 Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the BioBrick standard. It includes the native RBS, so it can be cloned directly in front of the desired expression target.


Source

The promoter Pcpc560 was discovered in 2013 by Jie Zhou et al1. For characterization studies, the gene was provided by Anna Behle2


References

1: Zhou, Jie, et al. "Discovery of a super-strong promoter enables efficient production of heterologous proteins in cyanobacteria." Scientific reports 4 (2014): 4500.

2: Behle, Anna, Pia Saake, and Ilka M. Axmann. "Comparative analysis of inducible promoters in cyanobacteria." bioRxiv (2019): 757948.